Entrez Gene: 5606 Human Entrez Gene: 26397 Mouse Entrez Gene: 303200 Rat Omim: 602315 Human SwissProt: P46734 Human SwissProt: O09110 Mouse Unigene: 514012 Human Unigene: 18494 Mouse Unigene: 100064 Rat
中文名称
磷酸化丝裂原活化蛋白激酶MKK3/6抗体
别 名
MKK3(Phospho-Ser218); MAP Kinase Kinase 3; Dual specificity mitogen activated protein kinase kinase 3; ERK kinase 3; MAP kinase kinase 3; MAP2K 3; MAPK ERK kinase 3; MAPK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK 3; MKK3; mMKK 3b; mMKK3b; MPK 3; PRKMK 3; PRKMK3; Protein kinase mitogen activated kinase 3; SKK2; zMKK 3; MP2K3_HUMAN; MP2K6_HUMAN
产品类型
磷酸化抗体
研究领域
肿瘤 信号转导 细胞凋亡 生长因子和激素 转录调节因子 激酶和磷酸酶 细胞膜受体
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
产品应用
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量
39kDa
细胞定位
细胞核 细胞浆 细胞膜
性 状
Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthesised phosphopeptide derived from human MKK3 around the phosphorylation site of Ser218:VD(p-S)VA
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed
PubMed
产品介绍
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Function:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Subunit:
Binds to DYRK1B/MIRK and increases its kinase activity. Part of a complex with MAP3K3, RAC1 and CCM2. Interacts with ARRB1. Interacts with Yersinia yopJ.
Tissue Specificity:
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Post-translational modifications:
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity. Phosphorylated by TAOK2.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
DISEASE:
Note=Defects in MAP2K3 may be involved in colon cancer.
Similarity:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
P46734
Gene ID:
5606
Database links:
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Sample:
Muscle(Rat) Lysate at 40 ug
Primary: Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) (bs-3274R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 40 kD
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-MKK3(Ser218) + MKK6(Ser207) (bs-3274R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 40 kD
Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-MKK3 (Ser218)+MKK6 (Ser207) Polyclonal Antibody, Unconjugated(bs-3274R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MKK3(Ser218) + MKK6(Ser207)) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
磷酸化丝裂原活化蛋白激酶MKK3/6抗体
作者:上海雅吉生物科技有限公司 2020-10-29T00:00 (访问量:1877)
上海雅吉生物科技有限公司 商家主页
地 址: 上海市闵行区元江路5500号第1幢5658室
联系人: 王源
电 话: 15301693058
传 真: 021-34661275
Email:yajikit@163.com
相关咨询
人原代附睾管上皮细胞 (2021-07-08T14:16 浏览数:10247)
雅吉生物热烈庆祝中国建党100周年 (2021-07-02T09:17 浏览数:5798)
15P-1 (小鼠睾丸上皮细胞) (STR鉴定正确) (2021-06-23T09:59 浏览数:8357)
客户细胞培养过程中常见的问题说明 (2021-06-18T09:54 浏览数:10514)
免疫荧光鉴定步骤 (2021-06-18T09:54 浏览数:11934)
人真皮成纤维细胞的分离培养制备方式 (2021-06-17T09:04 浏览数:8586)
原代角质形成细胞的分离培养方法 (2021-06-17T09:03 浏览数:12155)
钻石探头量子显微镜可以探索纳米微观世界的奥秘 (2021-06-16T09:24 浏览数:9280)
过氧化物酶体活化受体可以加剧肿瘤形成 (2021-06-16T09:23 浏览数:9094)
式细胞术数据分析之——圈门技巧 (2021-06-15T10:11 浏览数:9453)