EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher能提供全长重组人SMARCA2重塑酶。SMARCA2是ATP依赖性染色质重塑复合物SWI/SNF的核心ATP酶亚基，可动员核小体，在基因转录调控、发育、基因组复制和修复中发挥关键作用[1,2]。SWI/SNF亚基的异常活性与超过20%的人类癌症和多种发育障碍相关，这使得SMARCA2成为一个新兴的候选治疗靶点[3,4]。SMARCA2可用于结合EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201)在HTS兼容药物发现研究。

[1] Clapier & Carins Annu. Rev. Biochem. (2009). PMID: 19355820

[2] Sundaramoorthy & Owen-Hughes F1000Research (2020). PMID: 32864100

[3] Kadoch & Crabtree Sci. Adv. (2015). PMID: 26601204

[4] Cenik & Shilatifard Nat. Rev (2021). PMID: 32958894

产品详情

宿主来源: Human

表达系统: SF9 cells

保存温度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.

运输温度: Dry ice.

产品形式: SMARCA2 at 0.175 mg/mL in 20.7 µL of 25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin

验证数据

Figure 1: ATP-dependent chromatin remodeling assay EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 20 nM) incubated with SMARCA2 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.

Figure 2: Protein gel data SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

订购详情

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