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荧光素酶单克隆抗体

荧光素酶单克隆抗体

商家询价

产品名称: 荧光素酶单克隆抗体

英文名称: Firefly Luciferase

产品编号: hzm-33318M

产品价格: null

产品产地: 中国/上海

品牌商标: HZbscience

更新时间: 2023-08-17T10:24:20

使用范围: WB=1:500-5000

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 Mouse Anti-Firefly Luciferase antibody

 

产品编号 hzm-33318M
英文名称 Firefly Luciferase
中文名称 荧光素酶单克隆抗体
别    名 ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase.  
   
产品类型 标签抗体 
研究领域 激酶和磷酸酶  昆虫  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 11D6
交叉反应 Fruit Fly, 
产品应用 WB=1:500-5000 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 61kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Firefly Luciferase: 
亚    型 IgG1
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. 

Function:
Produces green light with a wavelength of 562 nm.

Subcellular Location:
Peroxisome.

Similarity:
Belongs to the ATP-dependent AMP-binding enzyme family.

SWISS:
N/A

Gene ID:
N/A

Database links:

SwissProt: P08659 Firefly (Photinus pyralis)



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Sample: Fruit Fly Lysate at 40 ug
Primary: Anti-Firefly Luciferase (bsm-33318M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD