荧光素酶单克隆抗体
产品名称: 荧光素酶单克隆抗体
英文名称: Firefly Luciferase
产品编号: hzm-33318M
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: WB=1:500-5000
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Mouse Anti-Firefly Luciferase antibody
产品编号 | hzm-33318M |
英文名称 | Firefly Luciferase |
中文名称 | 荧光素酶单克隆抗体 |
别 名 | ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase. |
产品类型 | 标签抗体 |
研究领域 | 激酶和磷酸酶 昆虫 |
抗体来源 | Mouse |
克隆类型 | Monoclonal |
克 隆 号 | 11D6 |
交叉反应 | Fruit Fly, |
产品应用 | WB=1:500-5000 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 61kDa |
细胞定位 | 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Firefly Luciferase: |
亚 型 | IgG1 |
纯化方法 | affinity purified by Protein G |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. Function: Produces green light with a wavelength of 562 nm. Subcellular Location: Peroxisome. Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. SWISS: N/A Gene ID: N/A Database links: SwissProt: P08659 Firefly (Photinus pyralis) Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Sample: Fruit Fly Lysate at 40 ug
Primary: Anti-Firefly Luciferase (bsm-33318M) at 1/2 000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD |