Disease:

 Esophageal squamous cell carcinoma 

Origin:

Established from poorly differentiated human esophageal squamous carcinoma 

Species:

Homo sapiens 

Tissue:

Esophagus 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Patient:

Medium:

DMEM + 10% FBS 

Subculture:

1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Related cells by Organ:

EC-109 cells 

HKESC-1 cells 

OE33 cells 

JROECL33 cells 

NE-1 cells 


References for HKESC-1 cells:

1. Hu, Y., Lam, K. Y., Wan, T. S., Fang, W., Ma, E. S., Chan, L. C., and Srivastava, G. Establishment and characterization of HKESC-1, a new cancer cell line from human esophageal squamous cell carcinoma. Cancer Genet Cytogenet, 118: 112-120, 2000.