CROP,环磷酸腺苷调节元件相关蛋白1抗体

产品编号HZ-14067R
英文名称CROP
中文名称环磷酸腺苷调节元件相关蛋白1抗体
别 名cAMP regulatory element-associated protein 1; Cisplatin resistance-associated overexpressed protein; Cisplatin resistance-associated-overexpressed protein; CRE-associated protein 1; CREAP-1; CREAP1; CROP; LC7L3_HUMAN; Luc7-like protein 3; Luc7A; LUC7L3; O48; Okadaic acid-inducible phosphoprotein OA48-18.
说 明 书0.1ml 0.2ml
研究领域细胞生物 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Pig, Cow, Sheep,
CROP,环磷酸腺苷调节元件相关蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量51kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CROP
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CROP,环磷酸腺苷调节元件相关蛋白1抗体PubMedPubMed
产品介绍background:
This gene encodes a protein with an N-terminal half that contains cysteine/histidine motifs and leucine zipper-like repeats, and the C-terminal half is rich in arginine and glutamate residues (RE domain) and arginine and serine residues (RS domain). This protein localizes with a speckled pattern in the nucleus, and could be involved in the formation of splicesome via the RE and RS domains. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2009]

Function:
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing.

Subunit:

Subcellular Location:
Nucleus speckle. The subnuclear localization is affected by cisplatin.

Tissue Specificity:
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.

CROP,环磷酸腺苷调节元件相关蛋白1抗体Post-translational modifications:
Phosphorylated in vitro by SRPK1, SRPK2 and CLK1. Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the Luc7 family.

Gene ID:
51747

Database links:
Entrez Gene: 51747 Human
Entrez Gene: 67684 Mouse
Entrez Gene: 360602 Rat
Omim: 609434 Human
SwissProt: O95232 Human
SwissProt: Q5SUF2 Mouse
Unigene: 130293 Human
Unigene: 30927 Mouse

CROP,环磷酸腺苷调节元件相关蛋白1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CROP Polyclonal Antibody, Unconjugated(HZ-14067R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining