FITC标记的腺苷单磷酸活化蛋白激酶γ2抗体
产品名称： FITC标记的腺苷单磷酸活化蛋白激酶γ2抗体
英文名称： Anti-PRKAG2/FITC
产品编号： HZ-9446R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-PRKAG2/FITC Conjugated antibody
FITC标记的腺苷单磷酸活化蛋白激酶γ2抗体
| 产品编号 | bs-9446R-FITC |
| 英文名称 | Anti-PRKAG2/FITC |
| 中文名称 | FITC标记的腺苷单磷酸活化蛋白激酶γ2抗体 |
| 别 名 | 5''-AMP-activated protein kinase subunit gamma-2; AAKG; AAKG2; AAKG2_HUMAN; AMPK gamma2; AMPKgamma2; AMPK subunit gamma 2; AMPK subunit gamma-2; CMH6; H91620p; Prkag2; Protein kinase AMP activated gamma 2 non catalytic subunit; WPWS. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 心血管 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRKAG2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: AMPK is a heterotrimeric complex comprising a catalytic a subunit and regulatory b and g subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKa1 and AMPKa2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKb1 encodes a 271 amino acid protein and human AMPKb2 encodes a 272 amino acid protein. The human AMPKg1 gene encodes a 331 amino acid protein. Human AMPKg2 and AMPKg3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. Function: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. Subunit: AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Subcellular Location: Cytoplasmic and Nuclear Tissue Specificity: Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis. Post-translational modifications: Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in PRKAG2 are the cause of cardiomyopathy, familial hypertrophic type 6 (CMH6) [MIM:600858]. A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH6 patients present Wolff-Parkinson-White ventricular preexcitation, enlarged myocytes without myofiber disarray, and glycogen-containing cytosolic vacuoles within cardiomyocytes. Defects in PRKAG2 are a cause of glycogen storage disease of heart lethal congenital (GSDH) [MIM:261740]; also known as phosphorylase kinase deficiency of heart or congenital nonlysosomal cardiac glycogenosis. GSDH is a rare disease which leads to death within a few weeks to a few months after birth, through heart failure and respiratory compromise. Similarity: Belongs to the 5'-AMP-activated protein kinase gamma subunit family. Contains 4 CBS domains. Database links: Entrez Gene: 51422 Human Entrez Gene: 108099 Mouse Entrez Gene: 373545 Rat Omim: 602743 Human SwissProt: Q9UGJ0 Human SwissProt: Q91WG5 Mouse Unigene: 647072 Human Unigene: 33649 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
AMPK是包含催化A亚基和调节B和G亚基的异三聚物复合物。它通过关闭ATP消耗的生物合成途径来保护细胞免于引起ATP耗竭的应激。AMPK通过一种涉及变构调节的机制激活高AMP和低ATP，通过被称为AMPK激酶的上游蛋白激酶促进磷酸化和抑制去磷酸化。活化的AMPK可以磷酸化和调节体内羟甲基戊二酰辅酶A还原酶和acetyl CoA carboxylase，它们是甾醇合成和脂肪酸合成的关键调节酶。人AMPKA1和AMPKA2基因分别编码548个氨基酸和552个氨基酸蛋白。人AMPKK1编码271个氨基酸蛋白，人AMPKK2编码272个氨基酸蛋白。人AMPKG1基因编码331个氨基酸蛋白。人AMPK2和AMPK3分别是569个和492个氨基酸蛋白，含有独特的N-末端结构域，并且可以直接参与AMPK复合物内AMP的结合。