FITC标记的HERC2 E3泛素蛋白连接酶抗体
产品名称： FITC标记的HERC2 E3泛素蛋白连接酶抗体
英文名称： Anti-HERC2/FITC
产品编号： HZ-15460R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-HERC2/FITC Conjugated antibody
FITC标记的HERC2 E3泛素蛋白连接酶抗体
| 英文名称 | Anti-HERC2/FITC |
| 中文名称 | FITC标记的HERC2 E3泛素蛋白连接酶抗体 |
| 别 名 | HECT domain and RCC1 like domain containing protein 2; Hect domain and RLD 2; jdf2; p528; SHEP1; HERC2_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 527kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HERC2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: HERC2 is a belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16. Function: E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. Subunit: Interacts (when phosphorylated at Thr-4827 and sumoylated) with RNF8 (via FHA domain); this interaction increases after ionizing radiation (IR) treatment. Interacts with XPA. Interacts with NEURL4. Via its interaction with NEURL4, may indirectly interact with CCP110 and CEP97. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole. Nucleus. Note=Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis. Post-translational modifications: Phosphorylation at Thr-4827 is required for interaction with RNF8. Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8. Similarity: Contains 1 cytochrome b5 heme-binding domain. Contains 1 DOC domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 1 MIB/HERC2 domain. Contains 19 RCC1 repeats. Contains 6 WD repeats. Contains 1 ZZ-type zinc finger. Database links: Entrez Gene: 8924 Human Omim: 605837 Human SwissProt: O95714 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
HERC2属于HERC基因家族，编码一组包含多个结构域的异常大蛋白。所有成员都具有至少一个N端区域的拷贝，该N端区域与细胞周期调节因子RCC1和C端HECT(与E6-AP C端同源)结构域相同，该结构域存在于许多E3泛素蛋白连接酶中。该基因的遗传变异与皮肤/头发/眼睛色素沉着变异有关。该基因的多个假基因位于染色体15和16。