重组人CD160蛋白（Recombinant Human CD160 Protein），GMP级

产品货号：GMP-TL708

产品描述（Description）

CD160 antigen is also known as natural killer cell receptor BY55, is a 27 kDa glycoprotein, contains 1 Ig-like V-type (immunoglobulin-like) domain. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. When expressed on vascular endothelial cells, CD160 propagates anti-angiogenic signals and promotes apoptosis.

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

分子别名（Synonym）

CD160、BY55、NK1、NK28

蛋白序列（Protein Sequence）

DNA序列编码人CD160（ GenBank: AAH14465.1）表达带有His标签在C末端

分子量（Molecular Weight）

重组人CD160蛋白 包含133个氨基酸，预测理论分子量为16.5 kD

纯度（Purity）

≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 HVEM(FC 标签)5μg/mL(100 μL/孔)能够结合人 CD160(His 标签)的线性范围是 2-50ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、CD160 is essential for NK-mediated IFN-γ production Tony C. Tu, Nicholas K. Brown, Tae-Jin Kim, Joanna Wroblewska, Xuanming Yang, Xiaohuan Guo, Seoyun Hyunji Lee, Vinay Kumar, Kyung-Mi Lee, Yang-Xin Fu J Exp Med. 2015 Mar 9; 212(3): 415–429. doi: 10.1084/jem.20131601 

2、CD160-Associated CD8 T-Cell Functional Impairment Is Independent of PD-1 Expression Selena Viganò, Riddhima Banga, Florence Bellanger, Céline Pellaton, Alex Farina, Denis Comte, Alexandre Harari, Matthieu PerreauPLoS Pathog. 2014 Sep; 10(9): e1004380. Published online 2014 Sep 25. doi: 10.1371/journal.ppat.1004380 

3、 CD160 isoforms and regulation of CD4 and CD8 T-cell responsesMohamed El-Far, Charles Pellerin, Louise Pilote, Jean-Francois Fortin, Ivan A D Lessard, Yoav Peretz, Elizabeth Wardrop, Patrick Salois, Richard C Bethell, Michael G Cordingley, George Kukolj J Transl Med. 2014; 12: 217. Published online 2014 Sep 2. doi: 10.1186/s12967-014-0217-y

  注意：For research and manufacturing use.