TrpRS抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
TrpRS抗体

TrpRS抗体

商家询价

产品名称： TrpRS抗体

英文名称： TrpRSAntibody

产品编号： XY 16081-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 TRPRS抗体Applications

In-house applications ELISA, WB, IHC, IF, FC, IP     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
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  • 16081-1-AP;HepG2 cellsHepG2 cells were subjected to SDS PAGE followed by western blot with 16081-1-AP(WARS antibody) at dilution of 1:500
  • 16081-1-AP;human ovary tumorImmunohistochemical of paraffin-embedded human ovary tumor using 16081-1-AP(WARS antibody) at dilution of 1:100 (under 10x lens)
  • 16081-1-AP;human ovary tumorImmunohistochemical of paraffin-embedded human ovary tumor using 16081-1-AP(WARS antibody) at dilution of 1:100 (under 40x lens)
  • 16081-1-AP;HepG2Immunofluorescent analysis of HepG2 cells, using WARS antibody 16081-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
  • 16081-1-AP;HepG2 cellsIP Result of anti-WARS (IP:16081-1-AP, 4ug; Detection:16081-1-AP 1:500) with HepG2 cells lysate 3000ug.
  • 16081-1-AP;HepG2 cells1X10^6 HepG2 cells were stained with 0.2ug WARS antibody (16081-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells
Positive IP detected in HepG2 cells
Positive IHC detected in Human ovary tumor tissue, human colon cancer tissue
Positive IF detected in HepG2 cells
Positive FC detected in HepG2 cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100
 TRPRS抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information TRPRS抗体
Immunogen TrpRS fusion protein ag9079 Full name Tryptophanyl-tRNA synthetase
Calculated molecular
weight		 471aa,53 kDa Observed molecular 
weight		 53 kDa
GenBank accession 
number		 BC017489 Gene ID (NCBI) 7453
Gene symbol WARS Synonyms GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, Tryptophanyl-tRNA synthetase, WARS, WRS
Background

TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing. TRPRS抗体

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